Summary of isoprene€‚ Emissions of isoprene, a reactive hydrocarbon, from Subarctic vegetation are not well documented. However, the Arctic is likely to experience the most pronounced effects of climatic warming, which may increase temperature-dependent isoprene emission.€‚ Here, we assessed isoprene emission from a Subarctic heath subjected to a 3–4°C° increase in air temperature and mountain birch (Betula pubescens ssp. czerepanovii) litter addition for  7–8 yr simulating climatic warming and the subsequent expansion of deciduous shrub species and migration of the treeline. The measurements were performed using the dynamic chamber method on a wet heath with a mixture of shrubs, herbs and graminoids. •  Isoprene emissions averaged across the treatments were  36 ± 5 µg m⁻² h⁻¹ in 2006 and 58 ± 7 µg m⁻² h⁻¹ in 2007.in 2006 and58 ± 7 µg m⁻² h⁻¹ in 2007 The experimental warming increased the emissions by 83% in 2007 (P = 0.021) and by 56% in 2006 (P = 0.056),while litter addition had no significant effects. The net ecosystem CO2 exchange was significantly decreased by warming in 2007. •  These results show that isoprene emissions from Subarctic heaths are comparable to those from Subarctic peatlands. Climatic warming will increase the emissions, and the amount of carbon lost as isoprene, from Subarctic heath ecosystems.