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Characterization of exopolysaccharides in marine colloids by capillary electrophoresis with indirect UV detection
Ansvarig organisation
2010 (Engelska)Ingår i: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 662, nr 2, s. 193-199Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A method was established using capillary electrophoresis with indirect UV detection for analysis of monosaccharides liberated from exopolysaccharides by acidic hydrolysis. Tangential flow filtration was used to isolate high molecular weight polysaccharides from seawater. The capillary electrophoresis method included the use of a background electrolyte consisting of 2,6-dimethoxyphenol and cetyltrimethylammonium bromide. Several neutral sugars commonly existing in marine polysaccharides were separated under optimized conditions. The relative standard deviations were between 1.3% and 2.3% for relative migration time and 1.3-2.5% for peak height. Detection limits (at S/N 3) were in the range of 27.2-47.8 mu M. The proposed approach was applied to the analysis of hydrolyzed colloidal polysaccharides in seawater collected from the Baltic Sea. Nanomolar levels of liberated monosaccharides in seawater samples can be detected by preconcentration up to 30,000 times. (C) 2010 Elsevier B.V. All rights reserved.

Ort, förlag, år, upplaga, sidor
2010. Vol. 662, nr 2, s. 193-199
Nyckelord [en]
Exopolysaccharide; Capillary electrophoresis; Extracellular polymeric substances; Marine colloid
Nationell ämneskategori
Naturvetenskap
Forskningsämne
SWEDARCTIC 2008, ASCOS
Identifikatorer
URN: urn:nbn:se:polar:diva-3093DOI: 10.1016/j.aca.2010.01.008OAI: oai:DiVA.org:polar-3093DiVA, id: diva2:1044929
Tillgänglig från: 2016-11-07 Skapad: 2016-10-27 Senast uppdaterad: 2017-11-29

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